ENA Panel

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Also known as: Antibodies to Saline-extracted Antigens; anti-RNP; anti-ribonucleoprotein; anti-SSA; SSA (Ro); anti-Sjögren Syndrome A; anti-SSB; SSB (La); anti-Sjögren Syndrome B; anti-Sm; Smith antibody; Scl-70; anti-topoisomerase; Scleroderma antibodies; anti-Jo-1
Formal name: Extractable Nuclear Antigen Antibodies
Related tests: ANA, Autoantibodies; Anti-dsDNA

At a Glance

Why Get Tested?

To help diagnose and distinguish between autoimmune disorders

When to Get Tested?

When your ANA (Antinuclear Antibody) test is positive; when you have symptoms that suggest an autoimmune disorder

Sample Required?

A blood sample drawn from a vein in your arm

Test Preparation Needed?


The Test Sample

What is being tested?

An ENA (Extractable Nuclear Antigen Antibodies) panel detects the presence of one or more specific autoantibodies in the blood. Autoantibodies are produced when a person's immune system mistakenly targets and attacks the body's own tissues. This attack can cause inflammation, tissue damage, and other signs and symptoms that are associated with an autoimmune disorder.

ENA are a subset of antinuclear antibodies (ANA), antibodies directed against proteins found in the nucleus of cells. Certain autoimmune disorders are characteristically associated with the presence of one or more extractable nuclear antigen antibodies. This association can be used to help diagnose an autoimmune disorder and to distinguish between disorders.

The ENA panel is typically a group of 6-10  autoantibody tests. The number of tests offered will depend on the laboratory and the needs of the doctors and patients it serves. ENA panel tests, and other less common ENA tests, may be able to be ordered separately depending on the laboratory.

The most common panel in Australia at present would include at least these antibodies:


Also Known As


Anti-U(1)RNP, Anti-Ribonucleoprotein


Smith Antibody

Anti-SS-A (Ro)

Anti-Sjögren Syndrome A

Anti-SS-B (La)

Anti-Sjögren Syndrome B


Scleroderma Antibodies; anti-topoisomerase


Antihistidyl Transfer RNA Synthase Antibodies

How is the sample collected for testing?

A blood sample is obtained by inserting a needle into a vein in the arm.

Is any test preparation needed to ensure the quality of the sample?

No test preparation is needed.

The Test

Common Questions

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NOTE: This article is based on research that utilizes the sources cited here as well as the collective experience of the Lab Tests Online Editorial Review Board. This article is periodically reviewed by the Editorial Board and may be updated as a result of the review. Any new sources cited will be added to the list and distinguished from the original sources used.