At a glance

Also known as

Ascitic fluid analysis

Why get tested?
To help diagnose the cause of peritonitis and/or peritoneal fluid accumulation (called ascites)
When to get tested?

When a doctor suspects that someone with abdominal pain and swelling, nausea, and/or fever has a condition associated with inflammation of the peritoneum (peritonitis) or ascites.

Sample required?
A peritoneal fluid sample obtained by inserting a needle into the abdominal cavity

What is being tested?

Peritoneal fluid is a liquid that acts as a lubricant in the abdominal cavity. It is found in small quantities between the layers of the peritoneum. Peritoneal fluid is produced by mesothelial cells in the membranes and acts to moisten the outside of the organs and to reduce the friction of organ movement during digestion.

A variety of conditions can cause accumulation of peritoneal fluid (ascites). The most common cause by far is cirrhosis of the liver (80% in Western Europe and north America).

Among the other causes are congestive heart failure, metastatic cancer, lymphoma, mesothelioma), rheumatoid disease, or systemic lupus erythematosus.

Laboratories use an initial set of tests (cell count, albumin and appearance of the fluid) in addition to the history and physical examination of the patient to help determine the cause of the accumulation of fluid.

How is the sample collected for testing?

A sample of peritoneal fluid is collected by a doctor with a syringe and needle using a procedure called paracentesis (see Common questions).
A sample of blood may be collected at the same time for measurement of serum albumin.

Is any test preparation needed to ensure the quality of the sample?

You will be asked to empty your bladder prior to sample collection.

The Test

How is it used?

Chemical tests - A Serum Ascites-Albumin Gradient  (SAAG) is calculated as the difference between the serum albumin concentration and the ascitic fluid albumin concentration. Tests that may be performed in addition to albumin may include:
Glucose - typically about the same as blood glucose levels but may be lower with infection.
Amylase - increased with pancreatic disease.

Microscopic examination - may be performed if infection or cancer is suspected. Normal peritoneal fluid has small numbers of white blood cells (WBCs) but no red blood cells (RBCs) or microorganisms.
Total cell counts - WBCs and RBCs in the sample are enumerated. Increased WBCs may be seen with infections and malignant conditions.
WBC differential - determination of percentages of different types of WBCs. An increased number of neutrophils may be seen with bacterial infections.
Cytology - a cytocentrifuged sample is treated with a special stain and examined under a microscope for abnormal cells and for white cell differentiation. The differential can help determine whether the cells are the result of an infection or the presence of a tumour.

Infectious disease tests - tests may be performed to look for microorganisms if infection is suspected.
Gram stain - for direct observation of bacteria or fungi under a microscope. There should be no organisms in peritoneal fluid.
Bacterial culture and susceptibility testing - ordered to detect any microorganisms, which will grow in the culture. If bacteria are present, susceptibility testing can be performed to guide antimicrobial therapy.
Other peritoneal fluid tests for infectious diseases that are less commonly ordered include tests for viruses, mycobacteria (AFB smear and culture) or parasites.

When is it requested?

Peritoneal fluid analysis may be ordered when a doctor suspects that a patient has a condition or disease that is causing peritonitis and/or ascites

What does the test result mean?

The common cause of a high SAAG value is cirrhosis of the liver but also other conditons e.g. congestive heart failure, cancer metastatic to the liver or carcinoma of the liver itself (hepatoma).

A low SAAG value may be found in peritonitis from connective tissue disorders, bowel perforation or infarction, in tuberculosis, pancreatitis and peritoneal malignancy.

Infectious diseases are caused by virusesbacteria, or fungi. Infections may originate in the peritoneum, be due to a rupture of the appendix, perforation of the intestines or the abdominal wall, contamination during surgery, or may spread to the peritoneum from other places in the body.

Is there anything else I should know?

A blood glucose may be ordered to compare concentrations with those in the peritoneal fluid. If a doctor suspects that a patient may have a systemic infection, then a blood culture may be ordered in addition to the peritoneal fluid analysis.

Common Questions

Are there other reasons to do a paracentesis?

Yes. Sometimes it will be performed to drain excess peritoneal fluid - to relieve pressure in the abdomen. The volume of fluid removed may be large - sometimes as much as four litres or more. This may need to be repeated periodically with some diseases.

Last Review Date: February 24, 2013